细胞培养基的选择

细胞培养基的选择细胞株名称ATCC编号细胞来源细胞种类生长状态使用培养基293CRL-1573人胎肾贴壁、上皮样MEM/NEAA,10%FBS2215无人肝癌贴壁、上皮样DMEM,15%FBS127TAgCRL-2817小鼠多能胚胎干细胞胚胎型DMEM,10%FBS293/CHE-FcCRL-2368人肾上皮细胞、贴壁DMEM,10%FBS3T3-L1CL-173小鼠胚胎成纤维成纤维DMEM,10%FBS3T6CCL-96小鼠胚胎成纤维细胞、贴壁DMEM,10%FBSA431CRL-1555人表皮细胞癌上皮细胞、贴壁DMEM,10%FBSA549CCL-185人肺癌贴壁、上皮样F12,10%FBSA9CRL-6319小鼠结缔组织成纤维细胞、贴壁DMEM,10%FBS+AR42JCRL-1492大鼠胰腺肿瘤贴壁、上皮样Ham'sF-12K,20%FBSAtT-20CCL-89小鼠垂体肿瘤上皮细胞、贴壁F-10,15%HS和2.5%FBSB95-8CRL-2311绒猴EB病毒感染的白血病细胞成淋样RPMI-1640,10%FBSBALB/3T3CCL-163小鼠胚胎成纤维细胞、贴壁DMEM,10%FBSbel7402无人肝癌贴壁、上皮样RPMI-1640,15%FBSBeWoCCL-98人绒毛癌上皮细胞、贴壁F-12K,15%F12BHK-21CCL-10仓鼠肾成纤维细胞、贴壁MEM/NEAA,10%FBSBHL-100无人乳房上皮细胞、贴壁McCoy'5A,10%FBS

BTCRL-1390牛鼻甲骨细胞成纤维细胞、贴壁DMEM,10%FBSCaco-2HTB-37人结肠腺癌上皮细胞、贴壁MEM/NEAA,10%FBSChang无人肝脏上皮细胞、贴壁BME,10%FCSCHO-K1CRL-9618仓鼠卵巢上皮细胞、贴壁F-12,10%FBSCL2CRL-2514小鼠B细胞淋巴细胞、悬浮RPMI-1640,10%FBSCL3CRL-2515小鼠B细胞淋巴细胞、悬浮RPMI-1640,10%FBSClone9CRL-1439大鼠肝脏上皮细胞、贴壁F-12K,10%FBSCloneM-3CCL-53.1小鼠黑素瘤上皮细胞、贴壁F-12,15%HS和2.5%FBSCOS-1CRL-1650猴肾成纤维细胞、贴壁DMEM,10%FBSCOS-3无猴肾成纤维细胞、贴壁MEM,10%FBSCOS-7CRL-1651猴非洲绿猴肾成纤维细胞、贴壁DMEM,10%FBSCRFKCCL-94猫肾上皮细胞、贴壁MEM/NEAA,10%FBSCV-1CCL-70猴非洲绿猴肾成纤维细胞、贴壁MEM/NEAA,10%FBSD-17CCL-183狗骨肉瘤上皮细胞、贴壁MEM/NEAA,10%FBSDANCRL-2130狗骨肉瘤上皮细胞、贴壁MEM/EBSS,8%FBSDaudiCCL-213人淋巴瘤病人血液成淋巴细胞、悬浮RPMI-1640,10%FBSDLD-1CCL-221人结肠直肠腺癌上皮细胞、贴壁RPMI-1640,10%FBSDSDhCRL-2131狗骨肉瘤上皮细胞、贴壁MEM/EBSS,8%FBSDSNCRL-9939狗骨肉瘤上皮细胞、贴壁MEM/NEAA,10%FBS

EB-3CCL-85人Burkitt淋巴瘤淋巴样、悬浮RPMI-1640,10%FBSES-D3CRL-1934小鼠多能胚胎干细胞胚胎型DMEM+15%FBSF9CRL-1720小鼠睾丸畸胎癌胚胎型至内胚层DMEM,15%FBSGH1CCL-82大鼠垂体肿瘤上皮细胞、贴壁F-12,15%HS和2.5%FBSGH3CCL-82.1大鼠垂体肿瘤上皮细胞、贴壁F-12,15%HS和2.5%FBSH9HTB-176人T细胞淋巴瘤成淋巴细胞、悬浮RPMI-1640,10%FBSH9/HTLV-IIIBCRL-8543人T细胞淋巴瘤成淋巴细胞、悬浮RPMI-1640,10%FBSHaKCCL-15仓鼠肾上皮细胞、贴壁BME,10%FCSHCT-15CCL-225人结肠直肠腺癌上皮细胞、贴壁RPMI-1640,10%FBSHelaCCL-2人宫颈癌贴壁、上皮样MEM/NEAA,10%FBSHelaS3CCL-2.2人子宫颈癌贴壁、上皮样F12,10%FBSHep-2无人喉癌上皮细胞、贴壁MEM,10%FBS;或RPMI-1640,10%FBSHep3BHB8064人肝癌贴壁、上皮样MEM/NEAA,10%FBSHepG2HB-8065人肝癌贴壁、上皮样MEM/NEAA,10%FBSHK-2CRL-2190人肾皮质贴壁、上皮样Keratinocyte-SerumFreeMedium+5ng/mlrhEGFHL-60CCL-240人急性早幼粒细胞白血病悬浮、粒细胞样RPMI-1640,20%FBSHP[HT1080poly]CRL-12012人纤维肉瘤上皮细胞、贴壁DMEM,10%FBSHT-1080CCL-121人纤维肉瘤上皮细胞、贴壁MEM/NEAA,10%FBS

HTCICLC收录ATL95006大鼠肝脏贴壁、上皮样MEM/EBSS,10%FBSHuT78TIB-161人T细胞淋巴瘤成淋巴细胞、悬浮IMDM,20%FBSHUVEC无人脐带内皮细胞、贴壁F-12K,10%FBS和肝素盐100ug/mlHX[HT1080xeno]CRL-12011人纤维肉瘤上皮细胞、贴壁DMEM,10%FBSI-10CCL-83小鼠睾丸癌上皮细胞、贴壁F-10,15%HS和2.5%FBSIEC-6CRL-1592大鼠正常小肠上皮样、贴壁DMEM,5%FBS,胰岛素0.1〃g/mlIM-9CCL-159人骨髓瘤病人骨髓成淋巴细胞、悬浮RPMI-1640,10%FBSIMR-90CCL-186人肺成纤维细胞、贴壁MEM/NEAA,10%FBSJAA-F11CRL-2381小鼠B细胞淋巴样、悬浮DMEM,10%FBS和NEAAJEG-3HTB-36人绒毛膜癌上皮细胞、贴壁MEM/NEAA,10%FBSJensenCCL-45大鼠肉瘤成纤维细胞、贴壁McCoy's5A,5%FBSJurkatTIB-152人急性T淋巴细胞白血病悬浮、淋巴细胞样RPMI-1640,10%FBSK-562CCL-243人骨髓性的白血病成淋巴细胞、悬浮RPMI-1640,10%FBSKBCCL-17人口腔癌上皮细胞、贴壁MEM/NEAA,10%FBSKG-1CCL-246人红白血病病人骨髓骨髓白细胞、悬浮IMDM,20%FBSKG-1aCCL-246.1人红白血病病人骨髓骨髓白细胞、悬浮IMDM,20%FBSL2CCL-149大鼠肺上皮细胞、贴壁F-12K,10%FBSL6CRL-1458大鼠骨骼肌成肌细胞贴壁DMEM,10%FBSLLC-WRC256CCL-38大鼠癌上皮细胞、贴壁199培养基，5%HSM.dunni(CloneIII8C)CRL-2017小鼠皮肤成纤维细胞、贴壁McCoy's5a,10%FBSMARCS5CRL-2507小鼠B细胞悬浮、淋巴细胞样MEM/NEAA或DMEM,20%FBS(灭活)MCF7HTB-22人乳腺癌上皮细胞、贴壁MEM/NEAA,10%FBS10ug/ml胰岛素MHCC97无人肝癌贴壁、上皮样DMEM,15%FBSMOLT-4CRL-1582人T淋巴细胞、急性淋巴细胞白血病悬浮、淋巴细胞样RPMI-1640,10%FBSNIH3T3CRL-1658小鼠胚胎贴壁、成纤维细胞DMEM,15%FBSNK-92CRL-2407人淋巴瘤成淋巴细胞、悬浮a-MEM,12.5%HS和12.5%FBSNK-92MICRL-2408人淋巴瘤成淋巴细胞、悬浮a-MEM,12.5%HS和12.5%FBSPANC-1CRL-1469人胰腺癌贴壁、上皮样DMEM,10%FBSPC12CRL-1721大鼠肾上腺嗜格细胞瘤多角型、松散贴壁、易结团生长F12,15%HS和2.5%FBSPC3CRL-1435人前列腺腺癌早期、骨转移贴壁、上皮样F12,10%FBSRajiCCL-86人Burkitt淋巴瘤淋巴样、悬浮RPMI-1640,10%FBSRat2CRL-1764大鼠Rat2(TK-)克隆衍生成纤维细胞样、贴壁DMEM,5%FBS,30pg/mlBrdURS4;11CRL-1873人急性淋巴细胞白血病成淋巴细胞、悬浮RPMI-1640,10%FBSSMMC7721无人肝癌贴壁、上皮样RPMI-1640,15%FBSSP2/0ACC146小鼠杂交瘤悬浮IMDM,7.5%FBSSW480CCL-228人结肠癌贴壁、上皮样10%FBS,L15SW620CCL-227人结肠癌贴壁、上皮样10%FBS+2mML-GLU+90%L15THP-1TIB-202人急性单核系白血病、血液悬浮、单核细胞样RPMI-1640,10%FBSU937CRL-1593.2人淋巴浆母细胞瘤悬浮、单核细胞样RPMI-1640,10%FBSVeroCCL-81非洲绿猴肾贴壁、上皮样DMEM,10%FBSVero-E6CRL-1586非洲绿猴肾贴壁、上皮样MEM/EBSS,10%FBSWEHI-3TIB-68小鼠骨髓单核细胞白血病类巨噬细胞、悬浮IMDM,10%FBSWI-38CCL-75人胚胎肺上皮细胞、贴壁BME,10%FBSWISHCCL-25人羊膜上皮细胞、贴壁BME,10%FBSWS1CRL-1502人胚胎皮肤上皮细胞、贴壁MEM/N