β-Apopicropodophyllin-DataSheet-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEβ-ApopicropodophyllinCat.No.:HY-N11600CASNo.:477-52-1分⼦式:C₂₂H₂₀O₇分⼦量:396.39作⽤靶点:Apoptosis作⽤通路:Apoptosis储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性β-Apopicropodophyllin⼀种天然产物，可以从Hyptiswticillata中分离得到。β-Apopicropodophyllin通过诱导微管破坏、DNA损伤、细胞周期停滞和ER应激来诱导细胞凋亡。β-Apopicropodophyllin可⽤于癌症研究。体外研究β-Apopicropodophyllin(0-100nM;48and72h)hasanti-canceractivityagainstA549,NCI-H1299andNCI-460celllineswithIC50valuesof16.9,13.1and17.1nM,respectively[1].β-Apopicropodophyllin(0-20nM;24and48h;A549,NCI-H1299andNCI-460celllines)disruptspolymerizationofintracellularmicrotubules[1].β-Apopicropodophyllin(0-20nM;0-24h;A549,NCI-H1299andNCI-460celllines)inducesDNAdamageviaactivationofATM,arrestscellcycleinG2/Mphaseandinducesendoplasmicreticulum(ER)stress[1].β-Apopicropodophyllin(15and20nM;48h;A549,NCI-H1299andNCI-460celllines)inducesapoptoticcelldeathinvitro[1].CellViabilityAssay[1]CellLine:A549,NCI-H1299andNCI-460cellsConcentration:0-100nMIncubationTime:48and72horsResult:Inhibitedcellgrowthinadose-dependentmanner.ApoptosisAnalysis[1]1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemECellLine:A549,NCI-H1299andNCI-460cellsConcentration:15and20nMIncubationTime:48hResult:IncreasedthepercentageofapoptoticdeathinA549,NCI-H1299andNCI-H460cellgroupsinatime-dependentmanner.CellCycleAnalysis[1]CellLine:A549,NCI-H1299andNCI-460cellsConcentration:0-20nMIncubationTime:8and16hResult:ArrestedcellcycleinG2/Mphase.WesternBlotAnalysis[1]CellLine:A549,NCI-H1299andNCI-460cellsConcentration:0-20nMIncubationTime:24and48horsResult:Decreasedthelevelofinsolubleproteincontainingpolymericmicrotubulesinatime-dependentmannerinnon-smallcelllungcancercelllines.WesternBlotAnalysis[1]CellLine:A549,NCI-H1299andNCI-460cellsConcentration:0-20nMIncubationTime:24hResult:IncreasedphosphorylatedATMandγ-H2AXlevelsinadose-dependentmanner.体内研究β-Apopicropodophyllin(1and5mg/kg;intra-tumorousinjection;nudemicewithxenografts)retardstumorgrowthinadose-dependentmanner[1].AnimalModel:nudemicewithxenografts[1]Dosage:1and5mg/kgAdministration:intra-tumorousinjection2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEResult:InhibitedtumorgrowthofNSCLCxenograftsinnudemice.REFERENCES[1].KimJY,et,al.Anovelanti-cancerroleofβ-apopicropodophyllinagainstnon-smallcelllungcancercells.ToxicolApplPharmacol.2018Oct15;357:39-49.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapp