血吸虫病和激动的肠炎的协会以结肠直肠的巨蟹星座：诊断和流行病学的Tools课件

TheAssociationofSchistosomiasisandInflammatoryBowelDiseasewithColorectalCancer:ToolsforDiagnosisandEpidemiologyPeterChun,Ph.DTheAssociationofSchistosomi1InEgypt,schistosomiasis(fromS.haematobiumandmansoni)hasbeenstronglylinkedtobladdercancer.Theevidenceincludes:EpidemiologicalevidenceExperimentallyinducedschistosomiasisHistopathologyAgeandgenderratiosM.H.Mostafaetal.RelationshipbetweenSchistosomiasisandBladderCancer.ClinMicrobiolRev1999.12(1)97-111TheevidencelinkingS.manosini&haematobiuminfectiontocancerofthelargeintestineisscant,butintestinalinfestationwithS.japonicumisconsideredasignificantcontributoryfactortothedevelopmentofcancerofthecolonandrectum.A.Ishiietal.Parasiticinfectionandcancer:withspecialemphasisonSchistosomajaponicuminfections(Trematoda).Areview.MutatRes.1994305(2)273-81InEgypt,schistosomiasis(fr2Colorectalcancercorrelateswithprevalenceandintensityofshistosomeinfection.Theageofonsetofcancerislowerinareasofshcistosomeendemicityandlengthofsurvivalisshorter.Affectedindividualsusuallyhavealonghistoryofinflammatorylargebowelsymptoms.Thecancerscorrespondinanatomiclocationtotheareamostaffectedbytheparasite.Z.Xuetat.Schsitosomajaponicumandcolorectalcancer:anepidemiologicalstudyinthePeople’sRepublicofChina.IntJCancer.1984.34(3):315-8C.Ming-Chaietal.Evolutionofcolorectalcancerinschistosomiasis:transitionalmucosalchangesadjacenttolargeintestinalcarconomaincolectomyspecimens>Cancer1980.46(7):1661-75Chenetal.Colorectalcancerandschistosomiasis.Lancet1981971-3ES.Zhaoetal.Carconomaofthelargeinstestinewithschistosomiasis—analysisof279cases.ZhonghuaZhongLiuZaZhi.19813(1):67-9ESZhao.Cancerofthecolonandschistosomiasis.JRSocMed.198174(9):645K.Shindo.Significanceofschistosomiasisjaponicainthedevelopmentofcancerofthelargeintestine.DisColonRectum1976;(19)460-9K.Inoguchietal.Thecorrelationbetweenprimarycolorectalcarcinomaandschistosomiasisjaponica.IgakkuKenkyu1978;(48)93-9K.Matsudaetal.PossibleAssociationsofRectalCarcinomawithSchistosomajaponicumInfectionandMembranousNephropathy:aCaseReportwithaReview.JapaneseJClinOncol(29)576-581

Colorectalcancercorrelates3Pathologically,patientswithcolonicschistosomiasismanifestepithelialdysplasia,andresemblethechangesfoundinlong-standingchroniculcerativecolitis.Chenetal.consideredthesedysplasticchangesaspremalignantlesions.Chenetal.Colorectalcancerandschistosomiasis.Lancet1981971-3TheevidenceassociatingcolorectalcancerandulcerativecolitisandCrohn’sdiseaseisstrong.Tumorsoftenoccuratthesiteofchronicinflammation.Theabsoluteriskofdevelopingcolorectalhankerishighwhenextensivediseasebeginsatayoungage.40%ofpatientsdiagnosedwithpancolitisfromulcerativecolitisbeforeage15yearsdevelopedcoloncancerduring20yearsoffollowupinapopulation-basedlinkagestudyinSweden.DKPodolsky.Inflammatoryboweldisease.NEnglJMed.2002.347:417-429Pathologically,patientswith4S.japonicumlaysaverylargenumberofegg,andrepresentsaconstantsourceofirritation.Inflammationinvolvesaseriesofhostreponsesthatisdesignedtopromotehealingandtissueregeneration.Theseincluderecruitingspecifictypesofcells,releaseofmediatorsandinteractionsofchemokineswithvarioustargetsthataffectcytologicalchanges.Leukocytesgeneratereactiveoxygenandnitrogenradicalswhichcandamageproteins,membranesandDNA(includingthegenescontrollingcellgrowth&proliferation).Inflammatorycellsalsoreleasefactorsthatinhibitapoptosis,induceangiogenesis,andimpaircertainimmuneresponses.Collectively,thesefactorscanacceleratemutagenesis,promotethesurvivalandclonalproliferationofmutatedcells,someofwhichmaybecomeinvasiveandmetastatic.

M.Thunetal.Inflammationandcancer:anepidemiologicalperspecitive.In2004CancerandInflammation.Wiley,Chichester(NovartisFoundationSymposium256).P6-28.S.japonicumlaysaverylarge5Wedescribeheretwomembranebasedcolloidalgoldtestswhichcanaidinthedetectionofinflammatoryboweldiseaseandschistosomiasis.Bothareeasytoperformandcanbedoneatthecliniclevelatthesametimethatotherscreeningtestsforcolorectalcanceraredone.WeproposetheuseofthesenewteststosecurelargescaleepidemiologicalevidencelinkingthesetwodiseasestocolorectalcancerinChinaWedescribeheretwomembrane6TestsforFecalOccultBloodChemicalTestsPrinciple:Basedonperoxidaseactivityofheme.Advantages:Sensitive,Inexpensive.Willdetectdigestedblood.Quantitationpossible.Disadvantages:Notspecific.Dietaryrestrictionrequired.ImmunologicalTestsPrinciple:Basedondetectionofhumanhemoglobin.Advantages:Veryspecific.Nodietaryrestrictions.Disadvantages:Expensive.Morecomplextoperfom.TestsforFecalOccultBloodCh7ExamplesofChemicalTestsChromogens:O-tolidine/Benzidine,Guaic,Pyramidon.ExamplesofChemicalTestsChro8ExampleofImmunologicalTestExampleofImmunologicalTest9血吸虫病和激动的肠炎的协会以结肠直肠的巨蟹星座：诊断和流行病学的Tools10Acomparisonwasmadeamongfourneutrophil-derivedproteinsinstoolasanindicatorofdiseaseactivityinulcerativecolitis.

Acomparisonwasmadeamongfo11Thereisaneedforarapidtestforlactoferrin.Animmunochromatographictestusingpolyclonalantibodies:asandwichimmunoassaywithcolloidalgoldasvisualizationreagent.Thereisaneedforarapidte12InstrumentswhichallowforquantitationInstrumentswhichallowforqu13InstrumentswhichallowforquantitationInstrumentswhichallowforqu14FlowthroughmembranetestfordetectingS.japonicumantibodiesTTestspotAntigenControlspotProteinACTestProcedure1.Wetmembrane2.AddserumFlowthroughmembranetestfor15FlowthroughmembranetestfordetectingS.japonicumantibodiesTC*

Specificanti-S.japonicumantibodiesarecapturedontheantigentestspot.*SerumantibodiesarenonspecificallycapturedonProteinAcontrolspotviaitsFcregion.0YYFcFlowthroughmembranetestfor16FlowthroughmembranetestfordetectingS.japonicumantibodiesTC3.Wash4.AddProtein-AGold5.WashYYFcAARedspotonTindicatesapositiveresult.Absenceofredspotindicatesnegative.RedspotonCindicatescorrectprocedure.Absenceindicateswrongprocedure.YY=Serumantibody(nonspecific)=Specificanti-S.japonicumantibodyProcedurecontinued…Flowthroughmembranetestfor17Thankyou

fromThankyoufrom18TheAssociationofSchistosomiasisandInflammatoryBowelDiseasewithColorectalCancer:ToolsforDiagnosisandEpidemiologyPeterChun,Ph.DTheAssociationofSchistosomi19InEgypt,schistosomiasis(fromS.haematobiumandmansoni)hasbeenstronglylinkedtobladdercancer.Theevidenceincludes:EpidemiologicalevidenceExperimentallyinducedschistosomiasisHistopathologyAgeandgenderratiosM.H.Mostafaetal.RelationshipbetweenSchistosomiasisandBladderCancer.ClinMicrobiolRev1999.12(1)97-111TheevidencelinkingS.manosini&haematobiuminfectiontocancerofthelargeintestineisscant,butintestinalinfestationwithS.japonicumisconsideredasignificantcontributoryfactortothedevelopmentofcancerofthecolonandrectum.A.Ishiietal.Parasiticinfectionandcancer:withspecialemphasisonSchistosomajaponicuminfections(Trematoda).Areview.MutatRes.1994305(2)273-81InEgypt,schistosomiasis(fr20Colorectalcancercorrelateswithprevalenceandintensityofshistosomeinfection.Theageofonsetofcancerislowerinareasofshcistosomeendemicityandlengthofsurvivalisshorter.Affectedindividualsusuallyhavealonghistoryofinflammatorylargebowelsymptoms.Thecancerscorrespondinanatomiclocationtotheareamostaffectedbytheparasite.Z.Xuetat.Schsitosomajaponicumandcolorectalcancer:anepidemiologicalstudyinthePeople’sRepublicofChina.IntJCancer.1984.34(3):315-8C.Ming-Chaietal.Evolutionofcolorectalcancerinschistosomiasis:transitionalmucosalchangesadjacenttolargeintestinalcarconomaincolectomyspecimens>Cancer1980.46(7):1661-75Chenetal.Colorectalcancerandschistosomiasis.Lancet1981971-3ES.Zhaoetal.Carconomaofthelargeinstestinewithschistosomiasis—analysisof279cases.ZhonghuaZhongLiuZaZhi.19813(1):67-9ESZhao.Cancerofthecolonandschistosomiasis.JRSocMed.198174(9):645K.Shindo.Significanceofschistosomiasisjaponicainthedevelopmentofcancerofthelargeintestine.DisColonRectum1976;(19)460-9K.Inoguchietal.Thecorrelationbetweenprimarycolorectalcarcinomaandschistosomiasisjaponica.IgakkuKenkyu1978;(48)93-9K.Matsudaetal.PossibleAssociationsofRectalCarcinomawithSchistosomajaponicumInfectionandMembranousNephropathy:aCaseReportwithaReview.JapaneseJClinOncol(29)576-581

Colorectalcancercorrelates21Pathologically,patientswithcolonicschistosomiasismanifestepithelialdysplasia,andresemblethechangesfoundinlong-standingchroniculcerativecolitis.Chenetal.consideredthesedysplasticchangesaspremalignantlesions.Chenetal.Colorectalcancerandschistosomiasis.Lancet1981971-3TheevidenceassociatingcolorectalcancerandulcerativecolitisandCrohn’sdiseaseisstrong.Tumorsoftenoccuratthesiteofchronicinflammation.Theabsoluteriskofdevelopingcolorectalhankerishighwhenextensivediseasebeginsatayoungage.40%ofpatientsdiagnosedwithpancolitisfromulcerativecolitisbeforeage15yearsdevelopedcoloncancerduring20yearsoffollowupinapopulation-basedlinkagestudyinSweden.DKPodolsky.Inflammatoryboweldisease.NEnglJMed.2002.347:417-429Pathologically,patientswith22S.japonicumlaysaverylargenumberofegg,andrepresentsaconstantsourceofirritation.Inflammationinvolvesaseriesofhostreponsesthatisdesignedtopromotehealingandtissueregeneration.Theseincluderecruitingspecifictypesofcells,releaseofmediatorsandinteractionsofchemokineswithvarioustargetsthataffectcytologicalchanges.Leukocytesgeneratereactiveoxygenandnitrogenradicalswhichcandamageproteins,membranesandDNA(includingthegenescontrollingcellgrowth&proliferation).Inflammatorycellsalsoreleasefactorsthatinhibitapoptosis,induceangiogenesis,andimpaircertainimmuneresponses.Collectively,thesefactorscanacceleratemutagenesis,promotethesurvivalandclonalproliferationofmutatedcells,someofwhichmaybecomeinvasiveandmetastatic.

M.Thunetal.Inflammationandcancer:anepidemiologicalperspecitive.In2004CancerandInflammation.Wiley,Chichester(NovartisFoundationSymposium256).P6-28.S.japonicumlaysaverylarge23Wedescribeheretwomembranebasedcolloidalgoldtestswhichcanaidinthedetectionofinflammatoryboweldiseaseandschistosomiasis.Bothareeasytoperformandcanbedoneatthecliniclevelatthesametimethatotherscreeningtestsforcolorectalcanceraredone.WeproposetheuseofthesenewteststosecurelargescaleepidemiologicalevidencelinkingthesetwodiseasestocolorectalcancerinChinaWedescribeheretwomembrane24TestsforFecalOccultBloodChemicalTestsPrinciple:Basedonperoxidaseactivityofheme.Advantages:Sensitive,Inexpensive.Willdetectdigestedblood.Quantitationpossible.Disadvantages:Notspecific.Dietaryrestrictionrequired.ImmunologicalTestsPrinciple:Basedondetectionofhumanhemoglobin.Advantages:Veryspecific.Nodietaryrestrictions.Disadvantages:Expensive.Morecomplextoperfom.TestsforFecalOccultBloodCh25ExamplesofChemicalTestsChromogens:O-tolidine/Benzidine,Guaic,Pyramidon.ExamplesofChemicalTestsChro26ExampleofImmunologicalTestExampleofImmunologicalTest27血吸虫病和激动的肠炎的协会以结肠直肠的巨蟹星座：诊断和流行病学的Tools28Acomparisonwasmadeamongfourneutrophil-derivedproteinsinstoo