小鼠药理实验分析

1、经小鼠初步毒理学实验确定致死量LD，得出LDo=1000mg/kg ,LD1oo=10000mg/kg。 在整个实验期间，雌、雄性小鼠分笼饲喂，定时饲喂标准全营养复合饲料，饲养室温度控制 在15-30C，相对湿度为60-85%，小鼠可自由采食并饮水自由。将40只小鼠雌雄分类随机分为5组。利用改良寇氏法26,27设计4组剂量组，剂量 的设置依据预实验得出的100%致死量LD100为最高值，按照组距等比递增计算得出（见表 1）。表1实验分组给药及检测血样情况分组小鼠个数（只）性别检测血样数给药肌注剂量（mg/kg ）剂量组分类110雄性51000最低剂量组210雄性52137低剂量组35雌性545

2、70高剂量组45雌性59772最高剂量组510雌性50空白对照组随机选取10只小鼠，雌雄各半，提取其血液样本。其血液的白细胞数目（WBCX红细胞数 目（RBC X血红蛋白（HGB X血小板数目（PLT）项目，相关数据如表1所示。本项研究 的小鼠HGB、RBC、WBC及PLT项目检测数据正常值参考范围分别为110-143（g/L）、 6.36-9.42（1012/L）、0.8-6.8（109/L）、450-1593（109/L）。将表 2 数据与其相对比可知，实 验前各小鼠血液指标均为正常值，且其外在活动也均表现正常。序号WBCRBCHGBPLT14.107.70129.00816.0024.5

3、07.53125.00898.0035.507.68137.00702.0045.408.42140.00833.0053.207.28136.00825.0064.606.80136.00788.0074.706.55128.00653.0085.307.65139.00850.0096.507.51118.00753.00105.406.82127.00825.00表3是根据实验分组给药的检测血样结果，其中第2组只抽检到4份血样。编组序号WBCRBCHGBPLT114.207.58115.00404.00124.304.7864.00489.00137.607.84115.00261.00

4、147.2010.59161.001169.001511.709.21130.00852.002612.109.85141.00833.00274.906.3295.00155.002813.709.54141.00960.002a99.607.44110.00662.003107.869.84138.001302.003118.808.71133.001361.003125.808.90138.001040.003137.809.23138.00931.003147.9010.05141.001218.0041517.608.60125.001129.004166.406.58103.008

5、31.0041710.808.92128.001414.0041825.308.72124.00595.0041911.608.50117.001189.005207.808.59124.001001.005219.7010.52165.001620.0052215.7010.38158.001173.005237.0010.08149.001083.005246.809.52136.001317.00一、单因素分析用SPSS Statistics 23对5个实验组进行单因素分析，看给药剂量对四个指标是否有显著性影响。操作步骤：1、导入数据WBCR6CHGBPLT114.2D7.63115.0

6、0仙仙口?124.304.73&4 00409 003137.6 D丁.84115.00261.0044.?2010.591&1.00115&0061S11.7D9.2113D.OO8 52.0 Of2&12.109 8SU1 00933 00274.9D6.3295.00155.OOfS2813.7D9.64141.00960.0Dfg299.ED7.4d11D.OO662.0 OfW3107.869.8413B.OO13Q2.0Df113118 8D8 71133 001361 OOF123125.8D8.9013B.OO104(.ODf13-3137 309.23130 00931 00

7、3147.SD10.06141.001215 001S41S17.609.60125 00112&00化4166.4 D6.闵103.00331.0041710 600 32129 001414 00I811825.3D8.72124.00S95.0DfF41911 &09.5011/001199 002D5207.8 D8.59124.00lootoof216219.7 D10.62165.00162fl.0Df2252215.7D10.3315B.OO1173.OOF235237.0 D10.03149.001083.ODf2452468D9 52136.0013170D2、工具栏，分析-

8、 比较平均值- 单因素ANOVA检验3、选择自变量和因变量4、点击事后比较，设置如下5、点击继续返回，点击选项，设置如下统计BI #疑酉固定和瞒机效应（E）BI方差齐性桂骏旧布朗辛塞斯担）韦尔奇堕）平均值圈r缺失值通按具体汾析排除个案色）成列排除个案（U继续笆）取消帮助6、点击继续，点击确定，得出输出结果。日回输出f日志 白.回单向标题| 备注 ! E描述 |方差商性检验 j“用 AN OVA aa事后植验r 口标题Ii奏重比较 白a汗性子集 口标题 |里WBC ffl| RBC |里HGB 引PLT输出结果分析描述个案牧平均值赫差揉准误差 海最小值瞪大伯FF艮质WBC 157.00003.0

9、66761.371503.192110.80794.2011.702410.07503.840461.920233.964016.18604.9013.70357.63201.10368.493586.26169.00245.808.804514.34007.311503.269805.261623.41846.4025.30559.40003.703381.656204.801713.99836.8015.70总计249.67334.76358.972367.661911.68484.2025.30RBC 158.00002.16464.968055.312210.68784.7810.59

10、248.28751.69307.846545.593410.98166.329.85359.3460.58235.260438.622910.06918.7110.05458.2640.95430.426777.07919.44896.588.92559.818078646.351728.841510.79458.5910.52总计248.76211.43163.292238.15769.36664.7810.59HGB 15117.000035.0784815.6875773.4443160.555764.00161.0024121.75(123.0560911.5280585.062615

11、8.437495.叩141.00351 37.60002.880971.28841134.0228141.1772133.00141.0045119.400010.014994.47884106.96471 31.83531D3.00128.00551 46.400016.562叩7.40675125.8356166.9644124.DD165.00总计24128.708322.185934.52868119.3400138.076664.00165.00PLT 15635.0000369.72895165.34782175.92091094.0791261.001169.0024652.50

12、00353 42750176.7137590.11801214.8820155.DD960.00351170.4000180.4364280.69362946.35861394.4414931.1361.00451031.6000320.68333143.41395633.41911429.7809595.0。1414.00551238.8000243.1978610876139936.8300154077001001.001620.00总计24957.8750375.4159876.63147799.35071116.3993155.001620.00由标准差可见，4个指标的组间离散情况是P

13、LTHGBWBCRBC。方盖齐性检验莱交统计自由度1自由度w显部WBC3.007419.041RBC2.127419.117HGB2.330419.088PLT.075419.497只有HGB的显著性小于0.05，方差不齐性，其他的显著性均大于0.05，说明方差齐性。ANOVA以方和自由度均方F显著性WBC 组间（组3 j166.477441.6192.225.105携性顼未加极40.035140.0352.143.160如枚42708142.7002.203.147偏差123.768341.2S62.205.121啪内355.4321910.707总计521.90923RBC 组间（组3 j

14、12.32543.0311.682.196携性顼未加极6.36616.3663.474.073如枚6.31816.3103.440.079偏差6.00632.0021.093.376啪内34.315191.332总计47.14023HGB 组间（组3 J3272.6084818.1521.931.147携性顼未加极1 554.44011 554.4403.670.071如枚1 547.95911 547.9593.654.071偏差1 724.6493574.8331.357.286啪内0048.35019423.597总计1 1 320.95823PLT 组间（组3 J1 541 862.4

15、254385465.6064.309.012携性顼未加极1223105.00011 2281 05.00013.723.002如枚1 1 00526.79511 108526.79513.206.002偏差353335.6303117778.5431.317.298啪内1 699692.2001909457.434总计3241 554.62523样本组间自由度为4，组内自由度为19，查F值界值表可知F表=3.564个指标只有PLT的F值F表，且sig0.05，说明PLT与剂量大小存在显著性差异。二、回归分析将对剂量有显著性差异的PLT做回归分析操作步骤：1、导入数据编组序号PLT剂量11404

16、.00100012489.00100013261.001000141169.00100015852.00100026833.00213721155.00213723960.00213729662.0021373101302.0045703111361.0046703121040.004670313931.0045703141218.0045704151129.009772416831.00卯724171414.009772418596.0097724191189.0097725201001.0005211620.0005221173.0005231083.0005241317.0002、工具栏点击分析-回归-曲线估算，设置如下，进行线性、二次和三次回归分析探索比较分析。3、点击确定，得到输出结果，对比发现三次回归的日方最大，残差最小，故建议采用三次 方拟合曲线做回归分析，回归方程为PLT=-1.598E-8刷量3-0.708*剂量+1209.882。三次模型摘要RR；