Stattic - STAT 抑制剂 - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEStatticCat. No.: HY-13818CAS No.: 19983-44-9分式: CHNOS分量: 211.19作靶点: STAT作通路: JAK/STAT Signaling; Stem Cell/Wnt储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 50 mg/mL (236.75 mM; Need ultra

2、sonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 4.7351 mL 23.6754 mL 47.3507 mL5 mM 0.9470 mL 4.7351 mL 9.4701 mL10 mM 0.4735 mL 2.3675 mL 4.7351 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保

3、存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (11.84 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (11.84 mM); Clear solutionBIOLOGICAL ACTIVITY1/2 Master of Small Molecules 您边的抑制剂师www.MedChemE物活性 Stattic有效的 S

4、TAT3 活化和聚化的抑制剂。IC50 & Target STAT3体外研究 Stattic inhibits STAT3 phosphorylation (Y705) in ALDH+ and D44+/CD24+ subpopulations of Panc-1 andHPAC pancreatic cancer cell lines at a higher concenration (20 M). Stattic selectively inhibits P-STAT3 asdemonstrated by the lack of inhibition of P-ERK1/2 in bot

5、h ALDH+ and CD44+/CD24+ subpopulations ofPanc-1 and HPAC. Stattic inhibita STAT3 downstream targets in ALDH+ and CD44+/CD24+ subpopulationsof pancreatic cancer cells 1. Stattic reduces the LPS-induced expression of ICAM-1 and VCAM-1, andSTAT3 phosphorylation 2.PROTOCOLCell Assay 1 Pancreatic cancer

6、stem-like cells (3,000/well in 96-well plates) are incubated with desired concentrations ofcompounds in triplicate at 37C for 72 h. MTT viability assays are done and the absorbance is read at 595nm.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表

7、的科研献 Cell Metab. 2019 Jan 8;29(1):141-155.e9. Gut. 2019 May 10. pii: gutjnl-2018-317424. Mol Cancer. 2019 Mar 30;18(1):64. J Immunother Cancer. 2019 Jul 12;7(1):178. J Immunother Cancer. 2018 Dec 18;6(1):151. 2018See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Lin L, et al. STA

8、T3 as a potential therapeutic target in ALDH+ and CD44+/CD24+ stem cell-like pancreatic cancer cells. Int J Oncol.2016 Oct 12.2. Cho YS, et al. Inhibition of STAT3 phosphorylation by sulforaphane reduces adhesion molecule expression in vascular endothelial cell.Can J Physiol Pharmacol. 2015 Nov 18:1-7.McePdfHeightCaution: Product has not been fully validated for medical applications