plus-DHMEQ-1R-2R-6R-Dehydroxymethylepoxyquinomicin-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemE(+)-DHMEQCat. No.: HY-14645ACAS No.: 287194-41-6Synonyms: (1R,2R,6R)-Dehydroxymethylepoxyquinomicin; (1R,2R,6R)-DHMEQ分式: CHNO分量: 261.23作靶点: Keap1-Nrf2作通路: NF-B储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 mont

2、h溶解性数据体外实验 DMSO : 35 mg/mL (133.98 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 3.8280 mL 19.1402 mL 38.2804 mL5 mM 0.7656 mL 3.8280 mL 7.6561 mL10 mM 0.3828 mL 1.9140 mL 3.8280 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 (+)-

3、DHMEQ种抗氧化转录因 Nrf2 的激活剂。(+)-DHMEQ是(-)-DHMEQ 的对映体。(+)-DHMEQ 抑制NF-kB 的活性低于(-)-DHMEQ。IC50 & Target Nrf2 11/2 Master of Small Molecules 您边的抑制剂师www.MedChemE体外研究 (+)-DHMEQ (2R,3R,4R)-DHMEQ) activates Nrf2, which is a transcription factor that induces the expressionof multiple antioxidant enzymes. (+)-DHMEQ

4、 activates Nrf2 in a promoter reporter assay. (+)-DHMEQ alsoincreases the expression of target antioxidant proteins and cancelled reactive oxygen species (ROS)-inducedcell death in a neuronal cell line. ROS generating 6-hydroxydopamine hydrochloride (6-OHDA) induces thedeath of SK-N-SH cells, and (+

5、)-DHMEQ decreases the cytotoxic effect of 6-OHDA, whereas its effect isweaker in Nrf2-knockdown cells prepared with siRNA. Thus, enhancement of the neural cell viability by (+)-DHMEQ is due to the activation of Nrf2 1.PROTOCOLCell Assay 1 SK-N-SH cells are seeded at 1.75104 cells/well in a 24-well p

6、late and cultured overnight. The cells aretreated with various concentrations of (+)-DHMEQ (1, 3, and 10 g/mL) for 24 h and subsequently treatedwith 300 M 6-OHDA for 24 h. Then, cells are stained with Trypan blue, and the number of stained cells iscounted 1.MCE has not independently confirmed the ac

7、curacy of these methods. They are for reference only.户使本产品发表的科研献 Sens Actuators B Chem. 2018 Nov 20;274:481-490. J Neuroimmune Pharmacol. 2019 Mar;14(1):94-109.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Niitsu Y, et al. Chemoenzymatic synthesis of (2R,3R,4R)-dehydroxymethylepoxyquinomicin (DHMEQ), a newactivator of antioxidanttranscription factor Nrf2. Org Biomol Chem. 2011 Jun 21;9(12):4635-41.McePdfHeightCaution: Product has not been fully validated for medical applications.For rese