Trolox - Reactive Oxygen Species - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemETroloxCat. No.: HY-101445CAS No.: 53188-07-1分式: CHO分量: 250.29作靶点: Reactive Oxygen Species作通路: Immunology/Inflammation; Metabolic Enzyme/Protease; NF-B储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实

2、验 DMSO : 100 mg/mL (399.54 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 3.9954 mL 19.9768 mL 39.9537 mL5 mM 0.7991 mL 3.9954 mL 7.9907 mL10 mM 0.3995 mL 1.9977 mL 3.9954 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清

3、的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (9.99 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (9.99 mM); Clear solution3. 请依序添加每

4、种溶剂： 10% DMSO 90% corn oil1/2 Master of Small Molecules 您边的抑制剂师www.MedChemESolubility: 2.5 mg/mL (9.99 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Trolox维素E类似物， 种强的抗氧化剂。体外研究 Trolox has shown to protect mammalian cells from oxidative damage. Trolox is effective in preventingmyocyte necrosis in cell cul

5、ture studies and in a canine model of two hours of left anterior descendingcoronary artery (LAD) occlusion followed by four hours of reperfusion 1. Trolox could prevent oxidativestress-induced apoptosis in thymocytes. Pre- or posttreatment of cells with Trolox reduced H2O2-inducedDNA fragmentation t

6、o control levels and below. 2. Trolox is a hydrophilic analogue of alpha-tocopherol andreported to scavenge peroxyl radicals better than vitamin E in sodium dodecyl sulfate micelles and inliposomes. Trolox prolongs substantially the survival of human ventricular myocytes and hepatocyte againstoxyrad

7、icals generated with xanthine oxidase plus hypoxanthine, and prevented lysis of red cells exposed toan azo-initiator 3.PROTOCOLCell Assay 1 Thymocytes are treated with Trolox (10 mM) before, during, or after 10 min exposure to 10 M H2O2. Troloxincubations that are conducted before or after H2O2 expo

8、sures are performed in TCM. Trolox treatments thatare administered concurrently with H2O2 are performed in PBS with ferrous sulfate 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Mickle DA, et al. Myocardial salvage with trolox and ascor

9、bic acid for an acute evolving infarction. Ann Thorac Surg. 1989 Apr;47(4):553-7.2. Forrest VJ, et al. Oxidative stress-induced apoptosis prevented by Trolox. Free Radic Biol Med. 1994 Jun;16(6):675-84.3. Wu TW, et al. The cytoprotective effect of Trolox demonstrated with three types of human cells. Biochem Cell Biol. 1990Oct;68(10):1189-94.McePdfHeightCaution: Product has not been fully validated for med