方便揿针针刺原始效应物质研究--ppt课件

1、针行方便揿针针刺原始效应物质的研究 Study on Acupuncture Original Material瘦美爆违尽环吉尿弥拌炭叮阐查苦碾斡肛庆盖蹭碍狙粗侯驮珐纠锰花昌梳方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究1ppt课件目的 将下述4项实验所取得的证据及电化学、微观腐蚀原电池效应科学规律，共同组成一个完整的证据锁链，证明针刺原始效应物质、针刺电化学效应机制。 Objective We will practice the following Four experiments made by the evidence, electrochemical and corrosi

2、on original micro-battery effect scientific laws, the common composition of a complete chain of evidence ，and will prove that the effect of acupuncture raw materials, electrochemical effects of acupuncture mechanism.孟玛峪秽举恕激视盐恼拯柔厄传默建笨沈崩柿钮纷磕吧戌怪毛脱雨龋斜陡方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究2ppt课件实验1 方便揿针针刺电化学电流研究实

3、验1-1 人体针刺电化学电流研究Experiment 1-1 Electrochemical current study by acupuncture黑筑离醚携磅希浑燕奸娱蛆乾赘誊琶峪茧愧雹轰慰仇舔念翻夷鞠阿阑卸胁方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究3ppt课件方法 将针灸针分别垂直刺入实验对象的肩髃、肩髎穴位中，用数字显示电流仪测量记录产生的电流变化Methods Acupuncture needles were stung vertically to alienate experiments of the points of Jianyu, Jian liao, wit

4、h figures current measurement records showed the changes of the current .绒膀讼撅杉陌昂益阔误缕难秋吻如袱灾淌佯丁侣弥也苗夺毅瞒投迪或帖辊方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究4ppt课件结果 不锈钢针灸针刺入人体穴位，所测的9名对象电流变化在-44 67A区间，电流差值为27 73A。Result Stainless steel acupuncture were needled into the human body , measuring the changes on the current targ

5、et is -44 67 uA interval, the current margin was 27 73 A on the cave of 9 cases .澄菏魄诱砧联曾膳荆棍有掐淹涣凭晃簇媚栅风暇毋蜜即氧拟途爸愿签丛棕方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究5ppt课件表1-1、针刺肩髃、肩髎穴的电流测定记录表（男=m、女=w）Table 1、 Statistics of electric current measured in the point of Jian Yu、Jian liao inserted（Man=m、Woman=w）N 性别 年龄（岁） 电流变化（A

6、） 电流差值（A） 峰值出现（次） Sex age（years） electric current electric current difference peak value 1 w 69 -2013 33 42 m 52 -943 52 53 w 65 -4418 62 54 m 78 -3716 53 6 5 w 75 -423 27 46 w 77 -667 73 47 m 72 -2448 72 58 w 54 -1239 51 59 m 23 -2547 72 6鸣捉梁驴严抵婉牡定霓胸戊趋箩俩煞籍拐绳巫帘讯飞学湍栅绷恤繁鸵虚予方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究

7、6ppt课件实验1-2 动物针刺电化学电流研究方法 将12只ICR实验小鼠按其存活及处死（强力颈椎脱位法）后的整个时间过程分为：A组（健康活体），B组（死亡1 h），及C组（死亡28 h）；先将针灸针分别刺入每只经麻醉的ICR实验小鼠左侧的曲池与后三里穴，用数字显示电流仪测量观察2min并记录产生的电流变化，后将该小鼠处死；待该小鼠处死1 h、 28 h后再分别对其右侧的曲池与后三里穴，并用数字显示电流仪测量观察2min并记录产生的电流变化；记录的数据再制表分析。音嘿鳃前鉴港鸵僧撑乍顿伏剂客焊梧哗炯斗码翔瞳党递咆燎杨枪思缓谦驭方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究7ppt课件

8、结果 针刺实验小鼠A组的所发电流在83 1A区间，并一直呈现由高向低的电流变化，电流发生率为100，电流的平均差值为35.92A； B组所发电流在41 -1A区间，并一直呈现由高向低的电流变化，电流发生率为100，电流平均差值为21.58A； C组所发电流在148 1A区间，一直呈现由高向低的电流变化，电流发生率为100，电流平均差值为68.92A。对睫独嘴债浩舀循峰赃新淹毛求植羔疫肿蚁么蓉骡撇口怜拆贩鬼舔顶酮予方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究8ppt课件表1-2、12只小鼠活时及死后针刺曲池、足三里穴所发电流发生率分析表（A）No 体重g A组 A组差值 B组 B组差

9、值 C组 C组差值1 19.4 286 22 2402412037 832 18.5 214 17 2332010243 593 19.1 362 34 9-11012367 564 21.2 369 27 34232438 355 18.3 152 13 167095913 466 20.0 832 81 383359618 787 18.3 588 50 416353812 268 19.7 344 30 3113014840 1089 17.9 618 53 122101121 11110 20.0 591 58 24222893 8611 17.3 233 20 1101110428

10、7612 18.5 282 26 265219330 63合计 228.2 831 431 41 -1 259148 1 827均数 19.02 35.92 21.58 68.92发生率 100 100100宰懦倒衡广捕迎越听领疵泌喇隐绞卵算宵沙貌用韧发兆逐借狮稍躇淆炕倦方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究9ppt课件分析 证明该实验所测电流为针刺电化学效应的依据如下：Analysis The current experiment provides for the evidence on the effects of acupuncture electrochemical.

11、褂虐均恃酶真微榔讣毙炽掩卜逾老腺绊罐描埂奉喝鄂惜哪汐柜擞琐传阐逮方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究10ppt课件A、由于无皮肤的组织电阻为300800，因此实验1-1所测到的-44 67A瞬间电流变化区间的电流差值为27 73A。而其瞬间电压变化区间（根据欧姆定律）应为：8.1V58.4V，明显远远大于50100mV的细胞动作电位；因此该瞬间电流不是我们已知的生物内在电流。而实验1-2所测的83 1A、41 -1A、148 1A瞬间电流变化区间的平均电流差值为35.92A、21.58A、21.58A，并一直呈现由高向低的电流变化，电流发生率为100，应与上述实验1-1的结

12、果相同。A、 The organization skin resistance was 300 to 800 cm on without skin, so the margin of transient current changes was 27 73 A on the interval -44 67 A. While the instantaneous voltage (according to Ohms law) was 8.1 V 58.4V, obviously far greater than the 50 100 mV of action potentials；As such,

13、the current was not biological internal currents as we know。驴往祥运菠己辽掳另罚锑景粹肿诗沾毯诸娥荤驴贝湃垂六矢惜魂种楔锌销方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究11ppt课件B、此外，笔者曾在2002年的离体动植物（猪肉、草莓）电化学测定实验中也明显证实了（结果与此次活体实验一样）其间也能产生uA级的电流。张大同.不同材质针灸针的电化学实验，上海针灸杂志，2003，22（5）：33-34. B、In addition ，the result also was proved by in vitro electroch

14、emical plants and animals of the experiment in 2002 (with the same vivo experiments) which could also have a Class A A Electricity. 张大同.不同材质针灸针的电化学实验.上海针灸杂志,2003.22（5）:33-34. 倡瓢萝字攀婆莎赦倾典菱凛装亿俺债弄辞淫享支塘聂俄窄锚阀呐琐都夏瓶方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究12ppt课件C、电化学科学规律。 C、The existing electrochemical universal laws o

15、f science.们岩钡堰钥蚊专氮挨术甜痔擎旺蜗弊门耀臂箱碱敷辑磊惜虹锑容屡占皿尤方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究13ppt课件实验1 结论 针刺穴位组织（能改变电位差）具有电化学原电池效应。Experiment 1 Conclusion Acupuncture points (to change the potential difference) have the original battery electrochemical effect.徊屹梗财国囚狮端浆夺够翼未兽讼股砧黔红胯绑溺冲茁雇帜檀发讥眩酒嚏方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究14

16、ppt课件实验2 针行方便揿针的腐蚀研究Experiment 2 Clinical studies of acupuncture needles corrosion（ The control group did not join because of original limited to the length, it was added as follows）桶嘱芒芬颓事犀庭负恋篷弯浦写桌扣剁镑围哟皇杉棚钩缄歇治贵练谨俘排方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究15ppt课件方法 将3600枚方便揿针针随机分成实验组（3000枚）和对照组（600枚），在一年中，实验组的300

17、0枚方便揿针在临床反复灭菌使用，对照组600枚方便揿针也随实验组一起反复灭菌但不使用，其间由目测挑选出具有可见腐蚀斑的方便揿针（数据采用x2检验处理，P0.05有统计学意义），再选典型腐蚀的方便揿针用电子显微镜放大拍摄分析。Methods 3,600 acupuncture needles were divided into the experimental group (3000) and the control group (600) randomly. 3000 acupuncture needles were used repeatedly by sterilization, and

18、600 acupuncture needles were sterilized again and again as the experimental group but without using，too. Meantime, selected corrosion spot with the acupuncture needle by the visual visible (data processing using x2 test, P 0.05 was significantly), the re-election of typical corrosion acupuncture nee

19、dles to be amplified with electronic microscope.宝罚稠函康诈甘锋桔映偶舍漠糜旺敖臃和纱囱妮惕飘漱辟侵沉彦老涅兼朗方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究16ppt课件结果 目测出实验组腐蚀针259枚（电子显微镜：腐蚀方便揿针表面呈现部分较严重腐蚀缺损区域），而对照组目测腐蚀针为0枚；实验组与对照组比较， P0.05差异有显著意义。请见具体资料。Result 259 needles were corroded in the experimental group (electron microscope: acupuncture ne

20、edle surface corrosion showed some of the more serious corrosion defect regions), while 0 needle was corroded in the control group by eyeballing; The result of experimental group compared with the control group, P 0.05 significant difference. See specific information.粉伏蜂抄拍缩囚蹬梦铆臼刚酪帛睡烽构器粥欣氖便戍狄粳嘛够茶儡禽渗撩

21、方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究17ppt课件表2、实验组方便揿针目测腐蚀率发生分析表Table 2、The experimental group stainless steel acupuncture needles visual analysis of corrosion rate in table 时间（月） 腐蚀针数 未腐蚀针数 分析总针数 腐蚀百分率Time（A month） Corrosion pin counts No corrosion pin counts The total number of needle Corrosion percentage1

22、3 0 3000 3000 046 19 2981 3000 0.6379 51 2930 2981 1.711012 189 2741 2930 6.45Total 259 2741 3000 8.63拂士降另拾萨啃蕴吭兄肆霹缝目幢俗班逆喉翻溯定虱觉瞳邯刁署挎狄太滔方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究18ppt课件表3、实验组与对照组目测方便揿针腐蚀率的比较Table 3、The experimental group and control group visual stainless steel acupuncture needle corrosion rate com

23、parison 腐蚀针数 未腐蚀针数 分析总针数 腐蚀百分率 Corrosion pin counts No corrosion pin counts The total number of needle Corrosion percentage实验组 259（8.63） 2741（91.37） 3000 8.63(The experimental group)对照组 0（0） 600 （100） 600 0(The control group)Total 259 3341 3600 7.19x 2=55.81562406 P0.05氓凿装分具谣匪沪邻驱宰卢纵揭诉磷锗勤杠哉葬吞佰戍搂畅匿酋请叹

24、羞逾方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究19ppt课件方便揿针的电子显微镜照片所示腐蚀现象 见图1、2、3Corrosion acupuncture needle shown in the electron microscope photographs of corrosion phenomenonSee figure 1, 2, 3辜葱疫鸯踏捕妖的扒糙屏捕斥叭载透夕广寞畴够客陕糙柿既腔呆刽蜡缮蝇方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究20ppt课件图1 放大50倍的腐蚀方便揿针局部Figure 1 Large 50 times the local corr

25、osion acupuncture needles碱灵稳荫蓟考重擎汕椭痪市樟密硒奠抗额占迅檬尘燥暂歼姆丝瑶恩讶斩磁方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究21ppt课件图2 放大100倍的腐蚀方便揿针局部Figure 2 Large 100 times the local corrosion acupuncture needles底鱼犁欣葡仇噶泉琉缉丽揣帘省律几姐房粘冈也什过雾兼砖寓值骂桓踌迎方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究22ppt课件图3 放大1000倍的腐蚀方便揿针局部Figure 3 Large 1000 times the local corr

26、osion acupuncture needles 奉难逗抖乳苟锅惺鲤算炼娇望耀渣娄加叭霉胜晴颅乡忠梆逊幢塌貌铣昆嫁方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究23ppt课件实验2 结论 针刺穴位组织会使针体腐蚀，该腐蚀现象符合电化学腐蚀原理。Experiment 2 ConclusionThe point of acupuncture needles willcorrode the acupuncture needles , corrosion phenomenon is consistent with the principle of electrochemical corro

27、sion.急糟郧之忿碘蔚琵卓川刀宅姚战盟捎令眼泻荣鱼碧昌饭呻抚辗抹胳疡罗舒方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究24ppt课件实验3 针刺穴位组织铬、镍、铁元素含量变化的研究Experiment 3 The study on the change of element of acupuncture Points with chromium, nickel, iron.柏葫况跺脱飞肺豺亦梳赫却兼浚犁刀苯撂透渔赏饺暴渔厅诅包锰抓奢漠键方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究25ppt课件方法 将15只实验兔按A（方便揿针10枚刺入左环跳穴区电针仪通电留针5h）、B

28、（方便揿针10枚刺入右臂臑穴区留针5h）实验组及C（右环跳穴区）、D（右臂臑穴区）对照组，进行实验，5h后对60个（每点15个）样本用(ICP-MS)电感耦合等离子体质谱仪（对与方便揿针主体相关联的铬、镍、铁元素的含量）进行分析，数据采用t检验处理，P0.05有统计学意义。Methods 15 rabbits were divided into the experimental groups and control groups ，the experimental groups were A (10 acupuncture needles piercing left Central Point

29、 district jump-electro-acupuncture needle telegram to stay 5 h) ，and B (10 acupuncture needles piercing right arm HDL Points district to stay 5 h). The control groups were C (right ring jump Point), and D (right arm HDL Point district. 60 samples (15 per point) were analyzed by electricity flu coupl

30、ed plasma mass spectrometer (with stainless steel acupuncture needles linked to the main chromium, nickel, iron content), data processing using t-test, P 0.05 with statistical significance.葵眶壁帝绪查覆洞艳凰瘟恭旋冕榷肝访报獭双衙专凿闺由像疡弗途拨邓爸方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究26ppt课件结果 实验组铬、镍、铁元素含量大幅升高，与对照组比较，P0.001其差异具显著意义。见表

31、4、表5。Result The experimental group chromium, nickel, iron content significantly increased, compared with the control group, P 0.001 with its significant difference. See Table 4 and Table 5.酥丘定蔑植绕井丢歪胁撩煽洱酒折酌救坷雌伸怔愚鬼素饵妓身悼院税酮岳方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究27ppt课件表4 两组臂臑穴区铬、镍、铁元素含量的比较 （S ，ng/g，各组n=15）Table

32、4、Compare of number of Cr、Ni、Fe in the point of Jian Yu between two groups 铬Cr 镍Ni 铁Fe实验组（留针） 270.0168271.63 116.1608743.87 27060.80411332.91 (stay needle)对照组Control 54.00709012.76 2.73888721.949 5523.45362380.729 Compared with the control group（与对照组比较）: P0.001傅冰皖卓炳何摸沉艰果家驼户鲤烫差苍粒乡芽坐垒妻银仿尹淫贬峦梳喇勺方便揿针针刺原

33、始效应物质研究方便揿针针刺原始效应物质研究28ppt课件表5 两组环跳穴区铬、镍、铁元素含量的比较（S ，ng/g，各组n=15）Table 5、 Compared with the element of Cr、Ni、Fe in the point of Jian liao between two groups 铬Cr 镍Ni 铁Fe实验组（电针） 303.93884115.7 134.7799164.55 14175.5915327.276(Electroacupuncture) 对照组Control 58.01403019.39 5.31042624.824 5414.27642531.7

34、67Compared with the control group（与对照组比较）: P0.001陨柳钮圃憎挚穿踪肝陕仟肢蔫凭朝震筋同各刊暇冰咒颇芒瞒幕组驴狸梅妈方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究29ppt课件实验3 结论 针刺穴位组织（能够增加离子、微量元素）具有电化学原电池效应。Experiment 2 ConclusionAcupuncture points (to increase ions, trace elements) have the original electrochemical battery effect.赚谆掘阑聪苹菠惦副穴恕溅仰术馁收一震凸摆炔小外紊粉蔷滥除削撂秆闲方便揿针针刺原始效应物质研究方便揿针针刺原始效应物质研究30ppt课件总体结论 按上述4项实验所取得的证据及电化学、微观腐蚀原电池效应科学规律，所共同组