常用荧光染料的激发及发射波长

1、实用标准文档荧光素名称激发波长 (nm)发射波长 (nm)备注1,5 IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA)5425685-FAM (5-Carboxyfluorescein)49251

2、85-HAT (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodamine)5785676045915-TAMRA (5-Carboxytetramethylrhodamine)5485425525686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hydroxy-4-me

3、thylcoumarin360449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471,474ABQ344445Acid Fuchsin540630ACMA (9-Amino-6-chloro-2- methoxyacridine)412, 430471,474Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine Yellow

4、470550Acriflavin436520Acriflavin Feulgen SITSA355-425460Aequorin (Photoprotein)466PhotoproteinAFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFPAlexa Fluor 350?346342442441Alexa Fluor 430?431540Alexa Fluor 488?495, 492519, 520Alexa Fluor 532?531, 532553, 554文案大全实用标准文档Alexa F

5、luor 546?556, 557572, 573Alexa Fluor 568?577, 578603Alexa Fluor 594?590, 594617, 618Alexa Fluor 633?632650Alexa Fluor 647?647666Alexa Fluor 660?668698Alexa Fluor 680?679702Alizarin Complexon530-560, 580580, 624645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Amino

6、methylcoumarin)345347425444AMCA-X353442Aminoactinomycin D555655Aminocoumarin346350442445Aminomethylcoumarin (AMCA)345347425444Anilin Blue600Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2+APTS424505Astrazon Bri

7、lliant Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO-TAG? CBQCA465560ATTO-TAG? FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9 (Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520Berberine S

8、ulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)381, 382,445, 447,blue shifted GFP 二文案大全实用标准文档Blue Fluorescent Protein383448(Y66H)Blue FluorescentProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+ 1Blan

9、cophor FFG390470Blancophor SV370435BOBO? -1462481BOBO? -31570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/5911584_592Bodipy 630/650-X625642Bodipy 650/6

10、65-X647665Bodipy 665/676605676Bodipy Fl504, 505511, 513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO? -1462481BO-PRO? -3544570Brilliant Sulphoflavin FF430520

11、BTC - Ratio Dye Ca2+464/401533/529Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+Calcein494517Calcein Blue373440文案大全实用标准文档Calcium Crimson?588, 589611,615Calcium Green501, 506531Calcium Green-1 Ca2+ Dye506|531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Calcium Green-5N Ca2+506532Ca2+

12、Calcium Green-C18 Ca2+509530Ca2+Calcium Orange549575576Calcofluor White385, 395,405437, 440,445Carboxy-X-rhodamine (5-ROX)576601Cascade Blue?377398399420423Cascade Yellow399400550552Catecholamine410470CCF2 (GeneBlazer)CFDA494520CFP - Cyan Fluorescent Protein430, 433,436, (453)474, 475,476, (501)Cyan

13、 Fluorescent ProteinCFP/YFP FRETChlorophyll480650Chromomycin A436-460470Chromomycin A445575CL-NERF (Ratio Dye, pH)504/514540Ratio Dye, pHCMFDA494520Coelenterazine Ca2+ Dye, bioluminescence(429)465Ca2+ Dye, bioluminescence, native moleculeCoelenterazine cp (Ca2+ Dye,)(430)442Ca2+ Dye, bioluminescence

14、Coelenterazine f(437)473Ca2+ Dye, bioluminescenceCoelenterazine fcp452Ca2+ Dye, bioluminescenceCoelenterazine h(437)464Ca2+ Dye, bioluminescenceCoelenterazine hcp(433)444Ca2+ Dye, bioluminescenceCoelenterazine ip441Ca2+ Dye, bioluminescenceCoelenterazine n(431)467Ca2+ Dye,文案大全实用标准文档bioluminescenceCo

15、elenterazine O460575Coumarin Phalloidin387470C-phycocyanineCPM Methylcoumarin384469MethylcoumarinCTC400-450602CTC FormazanCy2?489 n506Cy3.1 8554568Cy3.5?581598Cy3?514552554566570Cy5.1 8649666Cy5.5?675695Cy5?649666670Cy7?710, 743767, 805Cyan GFP433 (453)475 (501)cyclic AMP Fluorosensor (FiCRhR)500517

16、CyQuant Cell Proliferation Assay480520Cell Proliferation AssayDabcyl453Dansyl340578Dansyl Amine337517Dansyl Cadaverine335518Dansyl Chloride372518Dansyl DHPE336517Dansyl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DCFH (Dichlorodihydrofluorescein Diacetate)505535DDA

17、O463607DHR (Dihydorhodamine 123)505534Di-4-ANEPPS496705Di-8-ANEPPS (non-ratio)488498605713DiA (4-Di-16-ASP).456 口591文案大全实用标准文档Dichlorodihydrofluorescein Diacetate (DCFH)505535DiD - Lipophilic Tracer644665Lipophilic TracerDiD (DiIC18(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535DiI (DiIC18(3)549,

18、 551565Dinitrophenol349DiO (DiOC18(3)484, 487501, 502DiR748 二780Lipophilic TracerDiR (DiIC18)750779DM-NERF (high pH)497/510540Ratio Dye, pHDNP349Dopamine340490-520DsRed558583Red fluorescent proteinDTAF4941520DY-630-NHS621660Hemicyane label for proteins and DNADY-635-NHS634664Hemicyane label for prot

19、eins and DNAEBFP383447Enhanced BlueFluorescent ProteinECFP436474Enhanced CyanFluorescent ProteinEGFP488, 498507, 516Enhanced GreenFluorescent ProteinELF 97345530Eosin524545Erythrosin529, 532554, 555Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)5281617Euchrysin4305

20、40EukoLightEuropium (III) chlorideEYFP513, 520527, 532Enhanced YellowFluorescent ProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625文案大全实用标准文档FIF (Formaldehyd Induced Fluorescence)405433FITC490, 494520, 525FITC Antibody493517Flazo Orange375-530612Fluo-3480 -506, 506520, 527Fluo-4494516Fluo

21、rescein (FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1-43?479598FM 4-46515640Fura Red? (high pH)572657Fura Red?/Fluo -3Fura-2, high calcium335505Excitation ratio dyeFura-2, low calcium363512Excitation ratio

22、 dyeFura-2/BCECFGenacryl Brilliant Red B520590Genacryl Brilliant Yellow 10GF430485Genacryl Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type, non-UV excitation (wtGFP)475509GFP wild type, UV excitation (wtGFP)395509GFPuv385508Gloxalic A

23、cid405460Granular Blue355425Haematoporphyrin530-560580Hoechst 33258345487Hoechst 33342347483Hoechst 34580392440HPTS355465Hydroxycoumarin325-360386-455Hydroxystilbamidine (FluoroGold)361536Hydroxytryptamine400530ndo-1, high calcium330401Emission ratio dyendo-1, low calcium346475Emission ratio dye文案大全

24、实用标准文档Indodicarbocyanine (DiD)644665Indotricarbocyanine (DiR)748780Intrawhite Cf360 一430JC-1514 二529JO-JO-1530545JO-PRO-1532544LaserPro795812Laurodan355460LDS 751 (DNA)5431712LDS 751 (RNA)5901607Leucophor PAF370430Leucophor SF380465Leucophor WS395465Lissamine Rhodamine572, 577591, 592Lissamine Rhoda

25、mine B577；592LIVE/DEAD Kit Animal CellsCalcein/Ethidium homodimer494528517617for more details refer to LOLO-1566 二580LO-PRO-1568581Lucifer Yellow425, 428528, 536,540Lyso Tracker Blue373422Lyso Tracker Blue-White466536Lyso Tracker Green504, 534511, 551Lyso Tracker Red490516Lyso Tracker Yellow5511576L

26、ysoSensor Blue374424LysoSensor Green442505LysoSensor Yellow/Blue384540Mag Green507531Magdala Red (Phloxin B)5241600Mag-Fura Red483/427659/631Ratio Dye, Mg2+Mag-Fura-2369/329369/330508511/491Ratio Dye Ca2+Ratio Dye Mg2+Mag-Fura-5369/330369/332505/500505/482Ratio Dye, Ca2+Ratio Dye, Mg2+Mag-Indo-1349/

27、328349/330480/390480/417Ratio Dye, Ca2+Ratio Dye, Mg2+Magnesium Green506, 507531Magnesium Orange550575Malachite Green628Marina Blue3621459文案大全实用标准文档Maxilon Brilliant Flavin 10 GFF450495Maxilon Brilliant Flavin 8 GFF460495Merocyanin5551578Methoxycoumarin360410Mitotracker Green FM490516Mitotracker Ora

28、nge551576Mitotracker Red578599Mitramycin4501470Monobromobimane398 _n490Monobromobimane (mBBr-GSH)398500Monochlorobimane380461MPS (Methyl Green Pyronine Stilbene)364395NBD466 n539NBD Amine450530Nile Red515-555, 559590, 640Nitrobenzoxadidole465510-650Noradrenaline340490-520Nuclear Fast Red289-530580Nu

29、clear Yellow365495Nylosan Brilliant Iavin E8G460510Oregon Green503522Oregon Green 488-X494517Oregon Green?503522Oregon Green? 488490, 493514, 520Oregon Green? 500497517Oregon Green? 514506526Pacific Blue405455Pararosaniline (Feulgen)570625PBFI340/380420Excitation ratio dyePE-Cy5488670PE-Cy7488755, 7

30、67PerCP488675PerCP-Cy5.5488710PE-TexasRed Red 613488613Phloxin B (Magdala Red)524600Phorwite AR360430Phorwite BKL370430Phorwite Rev380430Phorwite RPA375430Phosphine 3R465565PhotoResist365 n610文案大全实用标准文档Phycoerythrin B PE546-565575Phycoerythrin R PE565578PKH26 (Sigma)551567PKH67496520ChromaPMIA341376

31、Pontochrome Blue Black535-553605POPO-1433457POPO-3533574PO-PRO-1435455PO-PRO-3539567Primuline410550Procion Yellow470600Propidium Iodid (PI)(305), 536, 538617PyMPO412, 415561, 564,570Pyrene360387Pyronine410540Pyronine B540-590560-650Pyrozal Brilliant Flavin 7GF365495QSY 7560Quinacrine Mustard440510Re

32、d 613 PE-TexasRed488613Resorufin571584, 585RH 414532716Rhod-2552576Rhodamine550573Rhodamine 110496, 497520Rhodamine 123507529Rhodamine 5 GLD470565Rhodamine 6G525555IRhodamine B540625Rhodamine B 200523-557595Rhodamine B extra550605Rhodamine BB540580Rhodamine BG540572Rhodamine Green502527Rhodamine Pha

33、llicidine558, 542575, 565Rhodamine Phalloidine542565Rhodamine Red570590Rhodamine WT530555Rose Bengal525, 540550-600文案大全实用标准文档R-phycocyanineR-phycoerythrin (PE)565578rsGFP473509red shifted GFP (S65T)S65A471504S65C479507S65L484510S65T488511Sapphire GFP395511SBFI340/380420Excitation ratio dyeSerotonin3

34、65520-540Sevron Brilliant Red 2B520595Sevron Brilliant Red 4G500583Sevron Brilliant Red B530590Sevron Orange440530Sevron Yellow L430490sgBFP?387450sgBFP? (super glow BFP)387450Quantum' s SuperGlo?GFP AFPssgGFP?474488sgGFP? (super glow GFP)474509Quantum' s SuperGlo?GFP AFPsSITS336436Ion Chann

35、elsSITS (Primuline)395-425450SITS (Stilbene Isothiosulphonic Acid)365460SNAFL calcein506/535535/620Ratio Dye, pHSNAFL-1508/540543/623Ratio Dye, pHSNAFL-2514/543546/630Ratio Dye, pHSNARF calcein552/574590/629Ratio Dye, pHSNARF1576/548635/587Excitation and emission ratio dyeSodium Green506, 507532Na+,

36、 K+SpectrumAqua433,/53480/55VysisSpectrumGreen497/30, 509/31538/44, 524/56VysisSpectrumOrange559/38, 560588/48VysisSpectrum Red587, 587/35612, 612/51SPQ (6-methoxy-N-(3- sulfopropyl)quinolinium)344443文案大全实用标准文档Stilbene335440Sulphorhodamine B can C520595Sulphorhodamine G Extra4701570SYTO 11SYTO 12508

37、, 510 :499, 500527, 530522, 519Dye for DNA, RNA 1 Dye for DNA, RNASYTO 13488, 491509, 514Dye for DNA, RNASYTO 14517, 521549, 547Dye for DNA, RNASYTO 15516, 518 :546, 555Dye for DNA, RNA SYTO 16488, 494518, 525Dye for DNA, RNA 1SYTO 17SYTO 18621490, 493634507, 527Dye for DNADye for DNA, RNASYTO 20512

38、530Dye for DNASYTO 21494517Dye for DNASYTO 225151535Dye for DNASYTO 23_499 1520Dye for DNA1SYTO 24490515Dye for DNASYTO 25521556Dye for DNASYTO 40420441Dye for DNASYTO 41430454Dye for DNA HSYTO 424331460Dye for DNASYTO 43436467Dye for DNAHSYTO 44446471Dye for DNASYTO 45452484Dye for DNASYTO 59622645

39、Dye for DNASYTO 606521678Dye for DNASYTO 61628 二645Dye for DNA HSYTO 62652676Dye for DNASYTO 63657673Dye for DNA二SYTO 64599619Dye for DNASYTO 80531 二545Nucleic Acid StainSYTO 81530 二544Nucleic Acid StainSYTO 82541560Nucleic Acid StainSYTO 83543559Nucleic Acid StainSYTO 84SYTO 85567567582583Nucleic A

40、cid StainNucleic Acid StainSYTOX Blue445470Nucleic Acid StainSYTOX Green5041523Nucleic Acid StainSYTOX Orange547570Nucleic Acid StainTetracycline390-425525-560Tetramethylrhodamine (TRITC)555576Texas Red?595620Texas RedX? conjugate595615文案大全实用标准文档Thiadicarbocyanine (DiSC3)651,653674, 675Thiazine Red

41、R596615Thiazole Orange510530Thioflavin 5430550Thioflavin S430550Thioflavin TCN350460Thiolyte370-385477-488Thiozole Orange453480Tinopol CBS (Calcofluor White)390430TMR550573TO-PRO-1515531TO-PRO-3644657TO-PRO-5747770TOTO-1514531, 533TOTO-3642660TriColor (PE-Cy5)(488) 650667TRITC TetramethylRodamineIsoThioCyanate550573True Blue365425T