Introduction of Circulating Tumor Cells detectionCTC检测的主要应用

1、 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Introduction of Circulating Tumor Cells detection1 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. 循环肿瘤细胞（CTC，Circulating Tumor Cell）是存在于外周血中的各类肿瘤细胞的统称，因

2、自发或诊疗操作从实体肿瘤病灶（原发灶、转移灶）脱落，大部分CTC在进入外周血后发生凋亡或被吞噬，少数能够逃逸并锚着发展成为转移灶，增加恶性肿瘤患者死亡风险2Circulating Tumor Cells 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. 早期诊断 术后/化疗后疗效评估 复发风险评估 预后评估 体内耐药性检测 指导个体化治疗CTC检测的主要应用3 2016 BD. BD, the BD Logo and all other tradema

3、rks are property of Becton, Dickinson and Company. CTC在PB中的比例太低CTC的半衰期短CTC的免疫分型指标复杂多变4CTC检测的难点 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. CTC在PB中的比例太低5CTC检测的难点 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson an

4、d Company. CD45磁珠负选。 注意事项：降低非特异染色。（死细胞、FC blocking，管子封闭。）6分离/富集CTC常用方法 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. CellSearch System7First and only FDA approved test for enumerating CTCs 2016 BD. BD, the BD Logo and all other trademarks are proper

5、ty of Becton, Dickinson and Company. DAPI+CD45-EpCAM+CK8/18/19+8CellSearch检测CTC使用指标 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Flexible-easy to add more markersAccurate-based on phenotypeCompatible-similar procedure with TBNK and B27Economical-no

6、 need to buy another expensive machineFACS检测CTC的优点9 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. 10FACS检测CTC的流程 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Workflow11Blood collectionPBMC isolatio

7、nLineage Cell DepletionStainingFACS detection and sortingIdentificationBD Vacutainer Venous Blood Collection BD Vacutainer CPT TubeBD IMag Human Lineage Cell Depletion SetBD PMG AntibodiesBD FACS/LSR analyzer and sorter 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickin

8、son and Company. 常用抗体12Antibodies特性特性CD45WBCEpCAM(CD326)上皮来源E-cadherin(CD324)CytokeratinsN-cadherin(CD325)间质来源VimentinALDH1*干性指标CD44CD24CD133*CD90 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. 测序/单细胞测序qPCR染色/单细胞染色CTC培养及功能验证后续实验13 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. 科研能力较强的医院或与医院密切合作的科研单位