组织与胚胎学实验：上皮组织

1、The epithelial Tissue Department of Histology and Embryology Classification of Epithelia Classification of Epithelia l1. Simple squamous epi. ( NO: 25) l2. Simple cuboidal epi( NO: 35) l3. Simple columnar epi. (NO: 1) l4. Pseudostratified ciliated columnar epi. (NO: 3) l5. Stratified squamous epi.(

2、NO: 2) l6. Transitional epithelium ( NO: 33) Requirement: Master and recognize the main types of covering epithelia. 1.Simple squamous epithelium( surface view) Material: frog mesentery Method: silver nitrate stain( Spread slide) Showpiece: Mesothelium surface view cell Irregular polygons Membrane a

3、nd extrocellular substance Mesothelium Nuclei are not stained, appears oval or rounded shape The light brown granular cytoplasm Simple squamous epithelium( lateral view ) Material: Stomach- Method: H&E Slide NO. 25 Mesothelium Cells are flat shapedoblate nuclei 2.Simple cuboidal epithelium Material:

4、 thyroid gland Method: H&E Slide NO. 35 Low power: lFollicle: many big and small follicles. The wall is consisted of simple cuboidal epi. lColliod High power: The cell is of square with a central round nucleus stained in purple blue. The cytoplasm is stained red. Note that the cells are as tall as t

5、hey are wide 3. simple columnar epithelium Material: human intestine Method: H&E Slide NO. 1 Low power： The outline of the villi is a line of the simple columnar epithelium 1 High power ： The outline of the villi is a line of the columnar cells. The oval nucleus is close to the base of the cell, sta

6、ined in blue . The cytoplasm is stained in red. Striated bonderGoblet cells Simple columnal epi. 4. pseudostratified ciliated columnar epi. Material: Human trachea Method: H&E Slide NO. 3 Low power： The inner surface of the wall is a line of the pseudostratif ied ciliated columnar epi. High power： 1

7、.Cilia 2.Columnar cell 3. Goblet cell. 4. Spindle cell 5.cone cell 6.Basement membrane cilia Basement membrane 1 2 3 4 5. Stratified squamous epithelium Material: human esophagus Method: H&E Slide NO. 2 Low power: The epi. is thick which lines the inner surface of the organ. It consists of many cell

8、 layers. 1. stratified squamous epithelium 1 High power: 1.squamous cell 2.polygonal cell 3.basal cell 1 2 3 surface intermediate basal Slide NO. 33 Transitional epithelium Material: Human urinary bladder Method: H&E 1.epithelium 1 1 Low magnification: The epithelium consists of many cell layers . M

9、iddle Magnification : Think of which functional condition does this specimen belong to ? 2 Middle cells layer Deep cells layer Surface cells domeshaped cells. Cytoplasm Nucleolus Cell Goblet cell Striated border simple columnar epithelium H&E stain 400 lWhat are the general features of epithelial tissue? 1) contain more cells and less extracellular ground substance 2) Polarisaton: -free outer surface: face the surface of the body or the lumen of an organ or gland -basal surface: face underlying connective tissue 3)