(考试资料下载)中南大学 生物化学期末考试试卷2004

中南大学2003-2004学年第二学期考试试卷Exam paper for the 2nd term of 2003-2004 at Central South UniversitySubject: BiochemistrySpecialty：BioengineeringCourse character：RequiredExam mode：Without reference documents but permission of any kind of dictionaryExam time length：120 minutesStudent Name_, Register No. _ Score_Part I True or False (1 point each， 20 pts) Please write T (true) or F (false) in the blanket1. Passive diffusion needs energy ( )2. Glucose transport in erythrocytes occurs by facilitated diffusion ( )3. All active transport systems are energy-coupling devices ( )4. Glycolysis is a catabolism to degrade glucose ( )5. Glycolysis provide the organism not only energy but also some important intermediates ( )6. TCA cycle is the major way to produce energy by aerobic organisms ( )7. Both photophosphorylation and oxdative phosphorylation produce ATP ( )8. ATP is not the major energy form in the living organism( )9. ATP, acetyl-CoA and glucose-6-phosphate have high-energy bond. ( )10. NADH normally occurs in biosynthesis pathway, while NADPH occurs primarily in biodegradation pathway ( )11. TCA cycle produces ATP directly ( )12. Carbohydrate produced by photosynthesis occurs in the dark period ( )13. Lipid bilayer takes part in mass transfer, energy production and signal communication ( )14. Biochemical reaction has not space but substrate specifications ( )15. Degradation of lipid can provide much more energy and water than carbohydrates ( )16. NADPH is necessary for maintaining the reductive environments for blood cells ( )17. Proline often occurs in a terminal and turn of a-helix of protein ( )18. Alpha helix and beta-sheet of protein is due to hydrogen bond, but subunits of hemoglobulin are arranged due to hydrophobic force. ( )19. Change in conformation is normally accompanied by breakage of chemical bond(s) ( )20. Pre-mRNA transcripted from DNA for prokaryotes normally does not need any processing to form the functional mRNA. ( )Part II Multiple Choice (1 point each， 30 pts) Choose the one best answer to each of the following questions.1. _ Biochemical synthesis of new cell material is calledA. MetabolismB. AnabolismC. CatabolismD. Synthatabolism2. _ TCA cycle is a pathway ofA. anabolismB. catabolismC. photosynthesisD. reductive pathway3. _ Chemoorganotrophs use which of the following as an energy source?A. Organic compoundsB. Inorganic compoundsC. Both organic and inorganic compoundsD. Light4. _ A fastidious organism isA. oxygen sensitiveB. temperature sensitiveC. nutritionally demandingD. atmospheric pressure sensitive5. _ If G0 is positiveA. the reaction is exergonic and requires the input of energy.B. the reaction in endergonic and requires the input of energy.C. the reaction is exergonic and energy will be released.D. the reaction is endergonic and energy will be released.6. _ Which statement is true?A. Coenzymes are generally bound tightly to their respective enzymes.B. Prosthetic groups are generally bound tightly to their respective enzymes.C. Coenzymes and prosthetic groups are both bound tightly to their respective enzymes.D. Coenzymes and prosthetic groups are technically not bound to any enzymes.7. _ In an electron carrier system, the net energy change is determined by the difference in reduction potentials between theA. primary electron donor and the terminal electron donor.B. primary electron acceptor and the terminal electron acceptor.C. primary electron acceptor and the terminal electron donor.D. primary electron donor and the terminal electron acceptor.8. _ The Embden-Meyerhof Pathway is another name forA. the citric acid cycleB. glycolysisC. electron transportD. NADH production9. _ In glycolysisA. the crucial product is ATP; the fermentation products are waste products.B. the crucial product is ethanol or lactate; ATP is a waste product.C. the crucial product is CO2; ATP is a waste product.D. the crucial product is not relevant because glycolysis is not a major pathway.10. _ In chemiosmosisA. OH- accumulates on the outside of the membrane while H+ accumulates on the inside.B. OH- accumulates on the inside of the membrane while H+ accumulates on the outside.C. both OH- and H+ accumulate on the inside of the membrane.D. both OH- and H+ accumulate on the outside of the membrane.11. _Which statement is not true? Uncouplers prevent ATP synthesisA. by interfering with electron flow.B. by creating leaky membranes.C. without affecting electron transport.D. by destroying the proton motive force.12. _ The chemical substance that enters the citric acid cycle for further metabolism is A. ethyl alcoholB. acetyl-CoAC. adenosine triphosphateD. pyruvic acid13. _ Which of the following occurs in the absence of oxygen?A. FermentationB. Anaerobic respirationC. Anoxygenic photosynthesisD. All of the above.14. _ Most chemolithotrophs areA. phototrophsB. autotrophsC. heterotrophsD. organotrophs15. _For a carbon source, chemoorganotrophs generally use such compounds asA. glucoseB. carbon dioxideC. triglyceridesD. sunlight16. _ For each molecule of pyruvate that enters the citric acid cycle, how many molecules of CO2 are generated?A. 1B. 2C. 3D. 617. _ Whether an organism is classified as a photoheterotroph or a photoautoroph depends on itsA. energy source.B. carbon source.C. oxygen requirements.D. All of the above.18. _ How many ATP result from the transfer of electrons from NADH to O2 through the electron transport chain?A. 1B. 2C. 3D. 619. _To describe a type of photosynthesis as oxygenic implies thatA. oxygen is producedB. oxygen is consumedC. oxygen functions as a catalyst20. _ The Calvin cycleA. is responsible for the fixation of CO2.B. utilizes only ATP.C. requires phosphofructokinase and pyruvate decarboxylaseD. all of the above21. _ DNA is always synthesized in the _ direction.A. amino terminal to carboxy terminalB. carboxy terminal to amino terminalC. 3 to 5D. 5 - 5E. 5 - 322. _ The short pieces of DNA made in the discontinuous strand are called _ fragments?A. IntronB. CistronC. ExonD. PolycistronicE. none of these23. _ DNA replication begins at locations called _ sites.A. PromoterB. OriC. Shine-DelgaroD. OkazakiE. Intron24. _ All DNA polymerases need a _ to attach a nucleotide to.A. RNA primerB. LigaseC. 3-OHD. deoxynucleotideE. 5-triphosphate25. _ Which of the following is not a substrate for DNA polymerases?A. deoxyadenosine triphosphateB. DNA templateC. a nucleotide primer with a free 3-OHD. deoxyuracil triphosphateE. deoxyguanosine triphosphate26. _ Which of the following replaces the RNA primer with deoxynucleotides?A. DNA polymerase IB. DNA polymerase IIC. DNA polymerase IIID. DNA ligaseE. primase27. _ If Vmax=100mmol/mL sec and Km=2mM, the velocity of the reaction is _, when S=20mMA. 108 mmol/mL secB. 91 mmol/mL secC. 67 mmol/mL secD. 145 mmol/mL sec28. _ The carboxylation catalyzed by a carboxylase usually needs a prothetic groupA. Fe2+B. biotinC. ferrocyaninD. Thiamine29. _ In a typical cycle of beta-degradation of C16 saturated fatty acids, it includes two steps of oxidation, in which the electron carriers are respectively :A. FAD and NAD+B. NAD+ and FADC. FAD and FADD. NAD+ and NAD+30. _In an erythrocyte undergoing glycolysis, what would be the effect of a sudden increase in the concentration of ATPA. Glycolysis inhibitedB. glycolysis stimulatedC. fructose-1,6-bisphosphate accumulatedD. acetyl-CoA accumulatedPart III Filling Blankets (1 point each, 20 pts)Please fill each blanket with suitable word(s) either in English or Chinese1. When external electron acceptors are not available in anoxic environments, carbon is catabolized by (_).2. The two major products of pentose phosphate pathway are (_) and (_)3. The major chemical energy forms from catabolism that are used for biosynthesis are (_) and (_).4. There are six subclasses of enzymes according to the Enzyme Commission, please give their systematic names (_; _,_, _;_; _)5. The fates of pyruvate produced by glycolysis are : (1)_; (2)_(3)_; (4)_6. The energy source for the heterotrophs, phototrophs and lithotrophs correspondingly are(_; _;_).7. Besides glycolysis, please list the other two most important pathways:(_; _)Part IV Short essay questions (30 pts)Please